Concanavalin A-mediated T cell proliferation is regulated by herpes virus entry mediator costimulatory molecule

T cell activation is regulated by two distinct signals, signals one and two. Concanavalin A (ConA) is an antigen-independent mitogen and functions as signal one inducer, leading T cells to polyclonal proliferation. CD28 is known to be one of major costimulatory receptors and to provide signal two in the ConA-induced T cell proliferation. Here, we have studied the implication of other costimulatory pathways in the ConA-mediated T cell proliferation by using soluble recombinant proteins consisting of an extracellular domain of costimulatory receptors and Fc portion of human IgG. We found that T cell proliferation induced by ConA, but not PMA plus ionomycin or anti-CD3 mAb, is significantly inhibited by herpes virus entry mediator (HVEM)-Ig, even in the presence of CD28 signaling. Moreover, the high concentration of HVEM-Ig molecules almost completely suppressed ConA-mediated T cell proliferation. These results suggest that HVEM might play more important roles than CD28 in ConA-mediated T cell proliferation.

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    Publication types

    Research Support, Non-U.S. Gov't

    MeSH terms

    CD28 Antigens / immunology* CD28 Antigens / metabolism Cell Proliferation / drug effects Concanavalin A / administration & dosage* Concanavalin A / immunology Herpesvirus 1, Cercopithecine / immunology Herpesvirus 1, Cercopithecine / metabolism Immunoglobulin Fc Fragments / immunology Immunoglobulin Fc Fragments / metabolism Immunoglobulin G / immunology Immunoglobulin G / metabolism Lymphocyte Activation / immunology Receptors, Tumor Necrosis Factor, Member 14 / immunology